Male sterile genes of Arabidopsis

Pollen development is critical for plant reproduction. Numerous nuclear mutations affect the function of pollen resulting in male sterility. The myb26 mutant is one such male sterile mutant allele, which results in anther indehiscence. Five putative MYB26 interactive proteins were identified from screening an Arabidopsis stamen yeast-2-hybrid library with MYB26 as bait. Förster Resonance Energy Transfer (FRET) was used to confirm the positive interaction between MYB26 and the Y2H320 protein. Analysis of the expression and possible function of the putative interactors was examined using SALK knockout T-DNA insertion mutants, RNAi and over-expression lines compared to the wild type (Ler). Using a Prom320::GUS transgene, GUS expression confirming that interaction in planta. Additional analysis of four Arabidopsis male sterile of novel mutants. Allelism analyses indicated that the two novel mutants are alleles of myb26, whilst another two novel mutations at different loci. Using (SSLP) molecular markers to identified a number of one of the novel gene of Gene mapping indicated that novel gene located on chromosome 1 that are related to male gametophyte and pollen development.